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second passage primary keratinocytes  (tebu-bio sa)


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    tebu-bio sa second passage primary keratinocytes
    Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult <t>keratinocytes.</t> ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.
    Second Passage Primary Keratinocytes, supplied by tebu-bio sa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+passage+primary+keratinocytes/pmc03912866-76-0-11
    Average 86 stars, based on 1 article reviews
    second passage primary keratinocytes - by Bioz Stars, 2026-09
    86/100 stars

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    1) Product Images from "Age-related changes in expression and function of Toll-like receptors in human skin"

    Article Title: Age-related changes in expression and function of Toll-like receptors in human skin

    Journal: Development (Cambridge, England)

    doi: 10.1242/dev.083477

    Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult keratinocytes. ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.
    Figure Legend Snippet: Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult keratinocytes. ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.

    Techniques Used: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    Poly (I:C) activates differential expression of chemokines, cytokines and angiogenic factors in fetal keratinocytes in comparison with neonates and adults. Keratinocytes from fetal, neonatal and adult donors ( n =3 donors/group) were cultured in KGM and stimulated with poly (I:C) or left untreated (negative control) for 24 hours. Supernatants were collected, pooled and analysed for the indicated chemokines, cytokines and angiogenic factors using commercially available protein arrays.
    Figure Legend Snippet: Poly (I:C) activates differential expression of chemokines, cytokines and angiogenic factors in fetal keratinocytes in comparison with neonates and adults. Keratinocytes from fetal, neonatal and adult donors ( n =3 donors/group) were cultured in KGM and stimulated with poly (I:C) or left untreated (negative control) for 24 hours. Supernatants were collected, pooled and analysed for the indicated chemokines, cytokines and angiogenic factors using commercially available protein arrays.

    Techniques Used: Expressing, Cell Culture, Negative Control

    Expression pattern of TLRs 3 and 6 in skin equivalents generated from fetal, neonatal and adult keratinocytes. ( A ) Cryostat sections of skin equivalents generated with keratinocytes from the indicated age groups ( n =3 donors/group), and adult breast skin (positive control), were stained for TLR 3 and 6 protein expression using immunohistochemistry. ( B ) Immunofluorescence double labeling revealed K10 − TLR3 + TLR6 + basal keratinocytes (red) and K10 + TLR3 − TLR6 − suprabasal keratinocytes (green). Nuclei were stained with Hoechst dye (blue). Scale bars: 50 μm in A,B (immunohistochemistry); 40 μm in B (immunofluorescence).
    Figure Legend Snippet: Expression pattern of TLRs 3 and 6 in skin equivalents generated from fetal, neonatal and adult keratinocytes. ( A ) Cryostat sections of skin equivalents generated with keratinocytes from the indicated age groups ( n =3 donors/group), and adult breast skin (positive control), were stained for TLR 3 and 6 protein expression using immunohistochemistry. ( B ) Immunofluorescence double labeling revealed K10 − TLR3 + TLR6 + basal keratinocytes (red) and K10 + TLR3 − TLR6 − suprabasal keratinocytes (green). Nuclei were stained with Hoechst dye (blue). Scale bars: 50 μm in A,B (immunohistochemistry); 40 μm in B (immunofluorescence).

    Techniques Used: Expressing, Generated, Positive Control, Staining, Immunohistochemistry, Immunofluorescence, Labeling

    Related Articles

    Cell Culture:

    Article Title: Age-related changes in expression and function of Toll-like receptors in human skin
    Article Snippet: Human dermal fibroblasts were obtained from Cascade Inc. (Portland, OR, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM, PAA, Linz, Austria) supplemented with 10% fetal bovine serum (FBS, PAA) and 1% penicillin/streptomycin (Gibco). .. Second passage primary keratinocytes from fetal (20-23 weeks EGA; n =3; tebu-bio, Offenbach, Germany), neonatal (3-4 days after birth; n =3; Lonza, Basel, Switzerland) and adult skin (19-37 years; n =3; Cell Systems, Troisdorf, Germany) were cultured in 12-well-plates (Costar, Cambridge, MA, USA) and grown to approximately 75% confluence in a serum-free, keratinocyte growth medium (KGM, Lonza) at 37°C and 5% CO 2 . .. Subsequent stimulation of cells was performed with the following heat-inactivated bacterial strains and TLR ligands (Invivogen, San Diego, CA, USA): Escherichia coli ( E. coli ; NK 9373) and Streptococcus pyogenes ( S. pyogenes ; clinical isolate), Pam3CSK4 (20 μg/ml), LTA-SA (10 μg/ml), poly (I:C) (low molecular weight; 20 μg/ml), native flagellin from Salmonella typhimurium and FSL-1 (10 μg/ml each).



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    second passage primary keratinocytes  (tebu-bio sa)
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    tebu-bio sa second passage primary keratinocytes
    Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult <t>keratinocytes.</t> ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.
    Second Passage Primary Keratinocytes, supplied by tebu-bio sa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+passage+primary+keratinocytes/pmc03912866-76-0-11
    Average 86 stars, based on 1 article reviews
    second passage primary keratinocytes - by Bioz Stars, 2026-09
    86/100 stars
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    Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult keratinocytes. ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.

    Journal: Development (Cambridge, England)

    Article Title: Age-related changes in expression and function of Toll-like receptors in human skin

    doi: 10.1242/dev.083477

    Figure Lengend Snippet: Bacterial stimuli and TLR ligands induce distinct immune responses in fetal, neonatal and adult keratinocytes. ( A ) Primary keratinocytes from fetal, neonatal and adult skin were cultured in 12-well plates to ~75% confluence and stimulated in triplicates for 24 hours with the indicated bacterial stimuli and ligands at concentrations described in the Materials and methods. IL1α was used as positive control, cell culture medium (untreated) and TSB as negative controls. ( B ) CXCL8 ( C ) CXCL10 and ( D ) TNFα levels in supernatants were quantified by ELISA. Experiments were performed as three biological replicates/age group (different donors) and the data represent the average (mean±s.e.m.) of these donors. * P <0.05, ** P <0.01, *** P <0.001. ( E ) Western blot analysis of untreated primary keratinocytes showed an almost equal expression of TLR3 and TLR6 proteins in all indicated age groups. Stimulation of keratinocytes with poly (I:C) did not affect TLR3 expression, whereas TLR6 expression was upregulated in fetal and neonatal, but not in adult, keratinocytes.

    Article Snippet: Second passage primary keratinocytes from fetal (20-23 weeks EGA; n =3; tebu-bio, Offenbach, Germany), neonatal (3-4 days after birth; n =3; Lonza, Basel, Switzerland) and adult skin (19-37 years; n =3; Cell Systems, Troisdorf, Germany) were cultured in 12-well-plates (Costar, Cambridge, MA, USA) and grown to approximately 75% confluence in a serum-free, keratinocyte growth medium (KGM, Lonza) at 37°C and 5% CO 2 .

    Techniques: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    Poly (I:C) activates differential expression of chemokines, cytokines and angiogenic factors in fetal keratinocytes in comparison with neonates and adults. Keratinocytes from fetal, neonatal and adult donors ( n =3 donors/group) were cultured in KGM and stimulated with poly (I:C) or left untreated (negative control) for 24 hours. Supernatants were collected, pooled and analysed for the indicated chemokines, cytokines and angiogenic factors using commercially available protein arrays.

    Journal: Development (Cambridge, England)

    Article Title: Age-related changes in expression and function of Toll-like receptors in human skin

    doi: 10.1242/dev.083477

    Figure Lengend Snippet: Poly (I:C) activates differential expression of chemokines, cytokines and angiogenic factors in fetal keratinocytes in comparison with neonates and adults. Keratinocytes from fetal, neonatal and adult donors ( n =3 donors/group) were cultured in KGM and stimulated with poly (I:C) or left untreated (negative control) for 24 hours. Supernatants were collected, pooled and analysed for the indicated chemokines, cytokines and angiogenic factors using commercially available protein arrays.

    Article Snippet: Second passage primary keratinocytes from fetal (20-23 weeks EGA; n =3; tebu-bio, Offenbach, Germany), neonatal (3-4 days after birth; n =3; Lonza, Basel, Switzerland) and adult skin (19-37 years; n =3; Cell Systems, Troisdorf, Germany) were cultured in 12-well-plates (Costar, Cambridge, MA, USA) and grown to approximately 75% confluence in a serum-free, keratinocyte growth medium (KGM, Lonza) at 37°C and 5% CO 2 .

    Techniques: Expressing, Cell Culture, Negative Control

    Expression pattern of TLRs 3 and 6 in skin equivalents generated from fetal, neonatal and adult keratinocytes. ( A ) Cryostat sections of skin equivalents generated with keratinocytes from the indicated age groups ( n =3 donors/group), and adult breast skin (positive control), were stained for TLR 3 and 6 protein expression using immunohistochemistry. ( B ) Immunofluorescence double labeling revealed K10 − TLR3 + TLR6 + basal keratinocytes (red) and K10 + TLR3 − TLR6 − suprabasal keratinocytes (green). Nuclei were stained with Hoechst dye (blue). Scale bars: 50 μm in A,B (immunohistochemistry); 40 μm in B (immunofluorescence).

    Journal: Development (Cambridge, England)

    Article Title: Age-related changes in expression and function of Toll-like receptors in human skin

    doi: 10.1242/dev.083477

    Figure Lengend Snippet: Expression pattern of TLRs 3 and 6 in skin equivalents generated from fetal, neonatal and adult keratinocytes. ( A ) Cryostat sections of skin equivalents generated with keratinocytes from the indicated age groups ( n =3 donors/group), and adult breast skin (positive control), were stained for TLR 3 and 6 protein expression using immunohistochemistry. ( B ) Immunofluorescence double labeling revealed K10 − TLR3 + TLR6 + basal keratinocytes (red) and K10 + TLR3 − TLR6 − suprabasal keratinocytes (green). Nuclei were stained with Hoechst dye (blue). Scale bars: 50 μm in A,B (immunohistochemistry); 40 μm in B (immunofluorescence).

    Article Snippet: Second passage primary keratinocytes from fetal (20-23 weeks EGA; n =3; tebu-bio, Offenbach, Germany), neonatal (3-4 days after birth; n =3; Lonza, Basel, Switzerland) and adult skin (19-37 years; n =3; Cell Systems, Troisdorf, Germany) were cultured in 12-well-plates (Costar, Cambridge, MA, USA) and grown to approximately 75% confluence in a serum-free, keratinocyte growth medium (KGM, Lonza) at 37°C and 5% CO 2 .

    Techniques: Expressing, Generated, Positive Control, Staining, Immunohistochemistry, Immunofluorescence, Labeling